Text GfV on the topic of SARS-CoV-2 PCR

There are always discussions on the use of the #PCR test as a diagnostic tool for #SARSCoV2. This text is therefore not aimed at experts, but is intended to give the general public an overview of the TOTAL process of #SARSCoV2 #diagnosis using PCR. We have therefore decided to describe the three major steps of PCR diagnostics for #SARSCoV2 from the #swab to the findings here: 1. pre-analytics (= preparation of the test sample) 2. technical analytics (= carrying out the PCR) and 3. post-analytics (= assessment of the PCR result).

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The Board of Directors of the Society of Virology

Prof. Dr. Ralf Bartenschlager, Heidelberg University Hospital

Prof. Dr. Thomas Stamminger, University Hospital Ulm

Prof. Dr. Ulf Dittmer, University Hospital Essen

Prof. Dr. Sandra Ciesek, University Hospital Frankfurt

Prof. Dr. Klaus Überla, Erlangen University Hospital

with the participation of:

Dr Marco Binder, German Cancer Research Center Heidelberg

Dr Sandra Bühler, Heidelberg University Hospital

Prof. Dr Isabella Eckerle, University Hospital Geneva, Switzerland

Prof. Dr. Markus Panning, University Hospital Freiburg

Jun. Prof. Dr. Stephanie Pfänder, Ruhr University Bochum

Sources

[1] https://www.ndr.de/nachrichten/info/57-Coronavirus-Update-Goldstandard-b...

[2] https://www.rki.de/DE/Content/InfAZ/N/Neuartiges_Coronavirus/Vorl_Testun...

[3] https://de.wikipedia.org/wiki/Polymerase-Kettenreaktion

[4] https://www.rki.de/DE/Content/InfAZ/N/Neuartiges_Coronavirus/Testzahl.html

[5] Lhopitallier L, Brahier T, Opota O, Kronenberg A, Mueller Y, Hugli O, et al. No evidence of SARS-CoV-2 circulation before identification of the first Swiss SARS-CoV-2 case. Int J Antimicrob Agents. 2020;56(3):106100.

[6] Opinion on INSTAND RV 340 SARS-CoV-2 NAT April 2020 of 29.09.2020 via https://www.instand-ev.de/

[7] https://www.ndr.de/nachrichten/info/57-Coronavirus-Update-Goldstandard-b...